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. 2015 Oct 28;10(2):272–281. doi: 10.1016/j.molonc.2015.10.007

Figure 3.

Figure 3

Endothelial Robo4 knockout led to increased tumor angiogenesis. (A) Left: H&E staining and IHC staining of tumor blood vessels (CD31) in E0.2 tumors of Robo4​+/+ and Robo4−/− mice. Right: Quantitative analysis of the CD31 staining areas (n = 3). (B) Flow cytometry analysis of relative numbers of tumor endothelial cells. Tumors from each group were digested into single‐cell suspensions using collagenase IV. Tumor endothelial cells (CD31+CD45−) were analyzed by flow cytometry. Statistical summary of 3 tumor samples from each group is shown in the bar graph of upper right panel. (*p < 0.05).