Figure 2.
FUS-mediated delivery of pBAL DNA-BPN across the BBB leads to robust and localized transgene expression in the rat brain. (A) Representative IVIS bioluminescence scans acquired 7 days after delivery of luciferase-bearing DNA-BPN into the rat brain with FUS. Bioluminescence was dependent on the DNA-BPN dose. (B) Ex vivo bioluminescence IVIS scans showing transgene distribution through axial plane (left) and coronal plane (right) 28 days after FUS treatment. (C) Representative IVIS bioluminescence images in a rat given 200 μg luciferase bearing DNA-BPN over 28 days. (D) Line graph of bioluminescence total flux over the 28 day test period. n = 5 at each dose. *Significantly different than all other doses tested (p<0.05).