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. 2015 Oct 8;15(2):445–461. doi: 10.1074/mcp.M115.051516

Fig. 5.

Fig. 5.

Identification of succination sites in VDAC2. A, B, Mitochondrial-enriched fractions from Ndufs4 KO mouse brainstem were resolved by SDS-PAGE, gels were stained with Coomassie Brilliant Blue, bands at ∼30–35 kDa were excised, destained and digested with trypsin as described under “Experimental Procedures.” MS/MS spectra showing that Cys77 in the peptide YKWC2SCEYGLTFTEK of VDAC2 is endogenously succinated in vivo (A); the unmodified Cys77 in the peptide WCPEEYGLTFTEK was also identified after alkylation with 4-vinylpyridine (B). C, D, MS/MS spectra showing that Cys48 in the peptide SC2SCSGVEFSTSGSSNTDTGK of VDAC2 is endogenously succinated in vivo (C); the unmodified Cys48 in the peptide SCPESGVEFSTSGSSNTDTGK was also identified after alkylation with 4-vinylpyridine (D).