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. 2015 Nov 23;15(2):573–597. doi: 10.1074/mcp.M115.051573

Fig. 10.

Fig. 10.

MALDI-TOF MS/MS of zwitterionic N-glycans. Ten example positive-ion mode MALDI-TOF MS/MS of N-glycans modified with (A) one aminoethylphosphonate (AEP) and one (B and C), two (D, E, F, and H) or three (G, I, and J) methylaminoethylphosphonate (MAEP) residues present in different RP-HPLC fractions (see Fig. 6B) are shown annotated with proposed structures for the individual fragments. Note that the glycans of m/z 1637 and 1961 are isobaric with standard oligomannosidic glycans (Man7,9GlcNAc2-PA). Digestion of a further methylaminoethylphosphonate-modified N-glycan (m/z 1151, isobaric with Man4GlcNAc2-PA) is shown in Fig. 11. An asterisk indicates modification of a Man or GlcNAc by methylaminoethylphosphonate.