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. 2015 Nov 24;15(2):715–725. doi: 10.1074/mcp.O115.054429

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 3. — Identification of AMPylation sites. (A) Structure of AMP-AzRTB derived adduct on Ser, Thr, or Tyr residues of AMPylated proteins and respective diagnostic ions formed during MS/MS analysis. (B) Exemplary annotated MS/MS spectra of high-confidence assignments of AMPylated peptides/sites (see Fig. S4 for other examples). MS/MS spectra for a given peptide were selected based on the highest probability score (-10 lgP) assigned by PEAKS7. Diagnostic ions are framed. The asterisk (*) denotes loss of a fragment of diagnostic ion with delta mass of 642.2018.