Table 1. Sequencing primers for the Lactobacillus species used in this study.

Species	Primer	Sequence (5ʹ-3ʹ)	Annealing temperature (°C)	Amplicon size (bp)
L. crispatus	LcrisF	AGCGAGCGGAACTAACAGATTTAC	58	154
	LcrisR	AGCTGATCATGCGATCTGCTT
L. gallinarum	LgallF	CGGTAATGACGCTGGGGAC	56	128
	LcrisR	AGCTGATCATGCGATCTGCTT
L. fermentum	LfermF	GCACCTGATTGATTTTGGTCG	58	317
	LactoR	GTCCATTGTGGAAGATTCCC
L. johnsonii	LactoF	TGGAAACAGRTGCTAATACCG	56	322
	LjohnR	CAGTTACTACCTCTATCTTTCTTCACTAC
L. salivarius	LsaliF	CGAAACTTTCTTACACCGAATGC	58	332
	LactoR	GTCCATTGTGGAAGATTCCC
L. acidophilus	F_acid	GAAAGAGCCCAAACCAAGTGATT	56	85
	R_acid	CTTCCCAGATAATTCAACTATCGCTTA
L. casei	F_case	CTATAAGTAAGCTTTGATCCGGAGATTT	56	132
	R_case	CTTCCTGCGGGTACTGAGATGT
L. delbrueckii	F_delb	CACTTGTACGTTGAAAACTGAATATCTTAA	56	94
	R_delb	CGAACTCTCTCGGTCGCTTT
L. paracasei	F_paca	ACATCAGTGTATTGCTTGTCAGTGAATAC	56	88
	R_paca	CCTGCGGGTACTGAGATGTTTC
L. plantarum	F_plan	TGGATCACCTCCTTTCTAAGGAAT	56	80
	R_plan	TGTTCTCGGTTTCATTATGAAAAAATA
L. reuteri	F_reut	ACCGAGAACACCGCGTTATTT	56	93
	R_reut	CATAACTTAACCTAAACAATCAAAGATTGTCT
E. faecalis	Efs130F	AACCTACCCATCAGAGGG	56	360
	Efs490R	GACGTTCAGTTACTAACG
Lactobacillus spp	F_alllact	TGGATGCCTTGGCACTAGGA	58	92
	R_alllact	AAATCTCCGGATCAAAGCTTACTTAT
All bacteria	F_eub	TCCTACGGGAGGCAGCAGT	58	466
	R_eub	GGACTACCAGGGTATCTAATCCTGTT