Fig. 4.

T cells, B cells, NK cells, and microglia/macrophages are present in the ipsilateral hemisphere following MCAO. At 96 h post-MCAO immunohistochemistry for immune cell surface markers shows peripheral immune cells are present in the ipsilateral hemisphere. Micrographs show CD3 positive cells (T cells) (a), CD161 positive cells (NK cells) (b), CD45R positive cells (B cells) (c), and CD11b positive cells (microglia/macrophages) (d) in the infarcted hemisphere. Micrographs from splenectomized rats demonstrate a decrease in immunostaining for T cells (e), NK cells (f), B cells (g), and microglia/macrophages (h) in the ipsilateral hemisphere. However in the contralateral hemisphere there is an absence of staining for T cells (i), NK cells (j), and B cells (k). Only microglia/macrophages were detected in the contralateral hemispheres (l). Inserts provide representative images of the morphological states of the microglia/macrophages present in each group and show an amoeboid cell (d), an amoeboid cell with evident ramifications (h), and a ramified cell (l). Scale bars=100 μm. The scale bar of the inserts=20 μm. Box in brain graphics depicts the regions where images were taken for a given micrograph