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. Author manuscript; available in PMC: 2016 Feb 13.
Published in final edited form as: Oncogene. 2014 Nov 24;34(33):4333–4346. doi: 10.1038/onc.2014.363

Figure 10. miR-146a loss results in increased proliferation in HCC1937 cells and clonogenic growth and mammosphere formation in HMLE cells.

Figure 10

A. Relative cell proliferation (as measured by the MTS Assay) of HCC1937 and HCC1937 WTBRCA1 cells transfected with scrambled and LNA miR-146a at 24 and 48 hrs after transfection. Results represent mean+/− SD of three independent experiments. B. Clonogenic images taken after 7-10 days of HMLE cells transfected with scrambled and LNA miR-146a are shown. Fold change is represented below the image. Data represent average of triplicates from three independent experiments. See also Fig.S6A,B,C. C,D&E. Western blots for BRCA1, EGFR, Raf, Ras, PARP and caspase 3 in HCC1937 cells after WTBRCA1 overexpression, EGFR knockdown and miR-146a mimic transfection. β-Actin was used as loading control. F&G. HMLE cells transfected with scrambled and LNA miR-146a and control and miR-146a mimic were cultured as primary and secondary mammospheres, and the number of spheres was counted per 1000 cells. Error bars denote +/− SD. ** p < 0.01, * p < 0.05 (Student's t test). (See also Figure S4). H, HCC1937 and HCC1937B cells transfected with scrambled and LNA miR-146a were cultured as primary mammospheres, and the number of spheres was counted per 1000 cells. Error bars denote +/− SD. ** p < 0.01, * p < 0.05 (Student's t test).