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. 2016 Jan 26;5:e09394. doi: 10.7554/eLife.09394

Figure 3. Host T cells are required for the progression of fibrosis.

Figure 3.

(A) Mallory staining of recipient RAG2KO organs shows that transplantation of mismatched BMSCs did not induce fibrosis in the absence of recipient T cells. Scale bar, 100 μm. (B) Tissue inflammation and excessive fibrosis in deep blue (*) was observed in BALB/c recipient mice after B10.D2 BMSC and RAG2KO HSC transplantation, despite the lack of donor T cells. Data collected from two replicate experiments (n = 3 per group). Scale bar, 100 μm. (A, B) D, duct; Ac, Acinus. (C): Significantly higher number of HSP47+ fibroblasts was observed in BALB/c recipients after B10 BMSC + RAG2KO HSC transplantation (red) compared to RAG2KO recipients after B10 BMSC + BALB/c HSC transplantation (blue). Data from five different fields in two replicate experiments. Data are shown as mean ± SD. *p<0.01, **p<0.001.

DOI: http://dx.doi.org/10.7554/eLife.09394.016

Figure 3—source data-1. Number of HSP47+ cells per field from the lacrimal gland, salivary gland, liver, and intestine.
Source data for graphs in (C). HSP, heat-shock protein.
DOI: http://dx.doi.org/10.7554/eLife.09394.017
elife-09394-fig3-data1.xlsx (27.6KB, xlsx)
DOI: 10.7554/eLife.09394.017