Figure 5. Adoptive transfer of recipient T cells from mismatched BMSC- transplanted recipients into BALB/c background Nude mice induce disease.
(A) Adoptive transfer of BALB/c T cells from mismatched BMSC-transplanted mice induced excessive fibrosis accompanied by numerous inflammatory cells in the lacrimal gland of naive nude mice, as shown by Mallory staining (excessive fibrotic area in deep blue [*]). Scale bar, 100 μm. Data collected from two replicate experiments (n = 4 per group). (B) The number of HSP47+ fibroblasts was significantly higher in various target organs following adoptive transfer of BALB/c T cells from mismatched BMSC recipients into nude mice (red), compared to wild-type (WT) nude mice (blue). Data are shown as mean ± SD. *p<0.005, **p<0.001. (C) CD4+ Th 17+ splenic cells were markedly elevated from adoptively transferred nude mice (left), compared to WT BALB/c background nude mice (right) (n = 4 each). (D) 1L-17 concentration in the serum from the same mice of the experiments shown in Figure 4C (n = 4 each). Data from one of two independent experiments (A,D). BMSC, bone marrow stromal/stem cells; HSP, heat-shock protein.
DOI: http://dx.doi.org/10.7554/eLife.09394.020
Figure 5—figure supplement 1. Adoptive transfer of recipient T cells from mismatched BMSC-transplanted recipients into BALB/c background Nude mice induce disease in target organs other than the lacrimal glands.
