Video 1. Typical raw data example showing the first 10,000 recorded frames from animal 5.
The left-hand side shows xy-corrected, but otherwise unaltered, raw fluorescence data. The legend above this raw data video shows the time after start of the experiment in seconds and the number of acquired frames. The top two panels on the right-hand side show (left) a depiction of the stimulation screen (gray isoluminant background or oriented drifting grating during stimulus presentation), and (right) whether the mouse is making a licking response. The two panels below show a live updated summary of mean dF/F0 (left) and heterogeneity (right) during each trial. Green indicates a licking response, and red indicates no response. The two lower panels show a live trace of mean population dF/F0 and heterogeneity. Licking responses are shown as red dotted lines, and stimulus presentations are shown as a gray shaded area. Note that the recording is very stable, except during periods of heavy licking, such as after hit responses, when reward is delivered. Also note that neural data acquired during licking are not used for any of our analyses and do, therefore, not influence our results. The mouse is licking vigorously during the initial period of the recording, but more typical behavior sets in less than 2 min after start of the recording. Near the end of the video, it can be seen that hits and misses are more easily separable using heterogeneity than dF/F0 (although this difference is stronger in the example video than in the entire data set as a whole).