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. Author manuscript; available in PMC: 2016 Feb 3.
Published in final edited form as: Exp Hematol. 2011 Jan 21;39(4):446–456. doi: 10.1016/j.exphem.2011.01.004

Figure 4. Urea Transporters mediate hydroxyurea transcellular movement.

Figure 4

(A–B) the apparent permeability coefficients of hydroxyurea transport from the apical to basal (A-to-B) direction and the basal to apical (B-to-A) direction is significantly higher for LLC-UTA cells (black bar) and LLC-UTB cells (striped bar) compared to LLC-pIRES control cells (white bar). (C–D) hydroxyurea transcellular transport over time in either direction is significantly higher for LLC-UTA cells (squares) and LLC-UTB cells (triangles) than for or LLC-pIRES cells (circles). (E–F) hydroxyurea transport measured in the presence of a competitive substrate inhibitor dimethylurea (DMU) is diminished in LLC-UTA cells (open squares) for both the A-to-B and B-to-A directions. (G–H) hydroxyurea transport with DMU is slightly diminished in LLC-UTB cells (open triangles) for both directions. Transport in control cells (open circle) is unaltered by DMU. All results represent mean ± SE of 2 independent experiments with 3 replicates of each experimental point. *** P<.001; **P<.01; * P<.05 compared to control without DMU.