Table 1.
Mean of transporter expression in CD71+ cells. HEK cell lines that over express each of the transporters (UTA, UTB, 1B1, and 1B3) were used as positive controls and HEK-pIRES cell line was used as a negative control. Values were normalized to β-actin and expressed as number of molecules × 105. For experiments with CD71+ cells, results are the average from 6 healthy volunteers (CD71+ cells) and 9 patients with sickle cell disease (CD71+ cells-SCD).
| Cell Type | UTA | UTB | OATP1B1 | OATP1B3 |
|---|---|---|---|---|
| CD71+ | 6 | 6208 | 0 | 0 |
| CD71+ SCD | 9 | 9938 | 0 | 0 |
| HEK-UTA | 44547 | 3 | 0 | 3 |
| HEK-UTB | 1849 | 3773 | 0 | 0 |
| HEK-1B1 | 2 | 0 | 228 | 0 |
| HEK-1B3 | 2 | 0 | 0 | 2211 |
| HEK-IRES | 5 | 0 | 0 | 0 |