Table 2.
Summary of the effect of buffer ionic strength on the release kinetics of 1, 2, and 3 from PEG-ASP CNAs when protected from light and after light exposure (photoactivated) in phosphate buffer (0 mM NaCl) and PBS (100 mM NaCl) (pH 7.4). Uncertainty expressed as standard error (N = 3).
| 1 | 2 | 3a | 1 | 2 | ||
|---|---|---|---|---|---|---|
| Dark | Photoactivated | |||||
| 0 mM NaCl | t1/2 (hrs) | 2.1 ± 0.2 | 8.8 ± 0.6 | 0.2 ± 0.07 (13.5 ± 4.8) | 9.9 ± 1.1 | 13.9 ± 1.8 |
| Release (%) | 90.0 | 77.0 | 45.2 (51.9) | 65.8 | 55.9 | |
|
| ||||||
| 100 mM NaCl | t1/2 (hrs) | 0.8 ± 0.03 | 3.9 ± 0.3 | 10.7 ± 2.4 | 3.0 ± 0.4 | 12.3 ± 2.7 |
| Release (%) | 98.7 | 92.4 | 37.0 | 77.7 | 52.6 | |
a
The release of 3 in solution with 0 mM NaCl was fit to a two-phase non-linear exponential regression. The first t1/2 value corresponds to release in the primary phase while the t1/2 value in parenthesis corresponds to release in the secondary phase. The first value for release indicates the percentage of 3 released in the primary phase while the value in parenthesis indicates the total percentage of 3 released in both phases.