Skip to main content
. 2016 Jan 29;7:10311. doi: 10.1038/ncomms10311

Figure 1. The nuclear location of effector fusion GFP–Pi04314 is important for its ability to promote infection.

Figure 1

(a) Images are projections of confocal Z series of GFP–Pi04314 and the forms of the fusion with added targeting signals; myrGFP–Pi04314 and NLSGFP–Pi04314 to decrease or increase the level of the effector fusion in the nucleus, respectively. Inset images are single optical sections through the nuclei of the cells. Scale bar, 20 μm. (b) GFP–Pi04314 and NLSGFP–Pi04314 are able to promote P. infestans growth following Agrobacterium-mediated expression compared with a GFP control. Lesion diameter was not significantly different between myrGFP–Pi04314-expressing plants and the control. Error bars are standard error and the graph represents the combined data from three biological replicates (n=56 per construct). Letters on the graph denote statistically significant differences (ANOVA, P≤0.005).