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. 2016 Jan 29;7:10509. doi: 10.1038/ncomms10509

Figure 7. The E2F/Rb pathway directly controls myogenic gene expression throughout development.

Figure 7

E2f1, E2f2, Dp and Rbf proteins are enriched upstream several myogenic genes in a Dp-dependent manner (a), and throughout development (b). Chromatin from pharate pupae (P96, a,b), third instar larvae (L3, b) and early pupae (P24, b) were immunoprecipitated with antibodies against Dp (top right panel), E2f1 (bottom left panel), E2f2 (bottom middle panel) or Rbf (bottom right panel), and compared with nonspecific control antibodies (top left panel). Muscle genes are how, fln, Lmpt, sals, Tm1, Mef2 and Tm2. The negative site does not contain predicted E2F-binding sites. The qPCR data are shown as fold enrichment relative to the negative site for each ChIP sample. Mean±s.d., n=2 replicates, two-way ANOVA, *P<0.05, **P<0.01, ***P<0.001. Genotypes are (a) Mef2-GAL4/UAS-mCherry-RNAi (white bar) and UAS-Dp-RNAi;Mef2-GAL4 (black bar), and (b) Mef2-GAL4/UAS-mCherry-RNAi.