Figure 2. Effects of suppression of Cav-1-F92A-HA expression and nitric oxide measurements.

A, Left panel, Whole lung protein from double transgenic mice was analyzed for the expression of the transgene at day 0, day 3 and day 7 after treatment with doxycycline (2 mg/ml) with 5 % sucrose in the drinking water. Each time point was repeated in three animals. Right panel, Densitometry analysis of the HA intensity and the HSP90 loading control. B, Left panels, Averaged systolic blood pressure from 8 control and 8 Cav-1-F92A animals. The first two days baseline was recorded, before drinking water was switched to doxycycline (2 mg/ml) with 5 % sucrose. Dotted lines show the changes of the systolic blood pressure (slopes of the curves are listed in Table I). Right panels, bar graph presentation of the same results. The bars present the average systolic blood pressure without and with doxycycline. C, Left panels, Averaged systolic blood pressure from 3 control and 3 Cav-1-F92A animals. The first two days baseline was recorded, before drinking water was switched to L-NAME (1 mg/ml). Dotted lines show the changes of the systolic blood pressure (slopes of the curves are listed in Table II). Right panels, bar graph presentation of the same results. The bars present the average systolic blood pressure without and with L-NAME. D, EPR-quantification of the peak to trough length of 4 mice each (representative traces are shown in Figure IV). E, Quantification of the p-VASP/t-VASP ratio (immunoblot is shown in Figure V).