SE induces neuronal damage in neocortex and thalamus. (A) Images of FJB staining in parietal cortex of Sham and SE24Hrs P7 pups. The image on the right of each row is a higher magnification of the boxed area on the image on the left. As shown, SE induces significant neuronal injury predominantly in layer 2 of the cortex. (B) Graph depicting extent of neuronal injury in parietal cortex, based on a 6-point scoring system. # p<0.05: SE 6 HRS vs. Sham, LiCl, Pilo (analyzed by Mann-Whitney). * p<0.05: SE 24 HRS vs. Sham, LiCl, Pilo (Mann-Whitney test). (C) Images of FJB staining in thalamus (lateral dorsal nucleus) of Sham and SE24Hrs P7 pups. The image on the right of each row is a higher magnification of the boxed area on the image on the left. SE results significant neuronal injury at 6 and 24 h post-SE. # p<0.05: SE 6 HRS vs. Sham, LiCl, Pilo (Mann-Whitney test). * p<0.05: SE 24 HRS vs. Sham, LiCl, Pilo, SE 6 HRS (Mann-Whitney test). Abbreviations: LD=lateral dorsal thalamic nucleus. Scale bars: (A, C) left column=100 µm (low magnification images) and right column 20 µm (high magnification images).