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. Author manuscript; available in PMC: 2017 Feb 2.
Published in final edited form as: Cell Rep. 2016 Jan 14;14(4):737–749. doi: 10.1016/j.celrep.2015.12.069

Figure 3. PSME3 Deficiency Impairs Bactericidal Activity of Macrophages.

Figure 3

(A) Effect of PSME3 deficiency on bactericidal activity in vitro. PEMs were incubated with E. coli at an MOI of 4, and bacterial killing capability was analyzed by APA. ***p < 0.001 (Student’s t test).

(B–D) Effect of PSME3 deficiency on LPS-induced expression of iNOS and CRAMP. PEMs were treated with L. monocytogenes (B), E. coli (C), or LPS (D) for 6 hr. The relative mRNA levels of iNOS and CRAMP was determined by quantitative real-time PCR. *p < 0.05; **p < 0.01 (Student’s t test). Ctrl, control.

(E) Effect of PSME3 deficiency on the protein level of iNOS. PEMs were stimulated with LPS for indicated hours. Cell lysates were analyzed by immunoblotting.

(F) Effect of PSME3 deficiency on NO production. Conditioned media of LPS-treated PEMs were analyzed for nitrite by the Griess assay. ***p < 0.001 (Student’s t test).

Error bars indicate mean ± SD.

See also Figures S2 and S3.