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. Author manuscript; available in PMC: 2017 Jan 8.
Published in final edited form as: Circ Res. 2015 Oct 26;118(1):56–72. doi: 10.1161/CIRCRESAHA.115.306874

Figure 4. Generation of human iPS-derived cardiac myocytes.

Figure 4

A. Timeline of human iPSC differentiation into late stage cardiac myocytes (days 0, 3, 5, 7, 14, 30, 60, 120, and 180) with corresponding gene activation as measured by PCR (n = 3). Error bars are standard deviation. Flow chart relates culture time with media, supplement, and cell type.

B. Immunofluorescence staining on differentiated cells isolated between days 30–60 for cardiac markers α-actinin, troponin T, and myosin heavy chain (MHC). Scale bars, 50 μm.

C. Flow cytometry analysis of a representative culture of human iPS-derived cardiac myocytes positive for α-actinin (left) and troponin T (right), isolated between days 30–60 post-differentiation.