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. Author manuscript; available in PMC: 2017 Jan 22.
Published in final edited form as: Circ Res. 2015 Dec 7;118(2):e19–e28. doi: 10.1161/CIRCRESAHA.115.307919

Figure 1. Basics of multimodal SHG-2PF imaging.

Figure 1

Jablonski diagrams help distinguish SHG and 2PF. Two photons excite a transition between states in both processes; the states are virtual in SHG and real in 2PF. Vibration reduces the energy of the excited state before 2PF emission. In contrast, no relaxation occurs with SHG. The schematic of our setup, detailed in the text, is depicted above.