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. Author manuscript; available in PMC: 2016 May 18.

Published in final edited form as: Oncogene. 2015 Aug 3;35(16):2087–2097. doi: 10.1038/onc.2015.271

miR-214-3p associates with survivin mRNA. A. Schematic representation for constructs of different survivin luciferase reporters containing the full length 3’UTR (FL-3’UTR), CR (F1), or 3’UTR fragments with individual predicted miR-214-3p binding sites (F2-F4). B. Luciferase activity in the different survivin reporter constructs following co-transfection with pre-miR-214-3p (50nM) or control miR in TE7 cells for 36 hours. Firefly luciferase activities were normalized to Renilla luciferase activities and expressed as the mean of three independent experiments, where all experiments were carried out in triplicate. Error bars represent mean ± S.D. and * represents statistically significant p < 0.05, p values based on two-tailed Student's t test. C & D. The binding sequence of the miR-214-3p potential binding site in survivin 3’UTR fragments F2 (C) and F4 (D) were mutated (schematic, left top and bottom) by substituting 4 bases (underlined). Luciferase activity was measured and compared with its WT fragment. E. Binding sequences of both the specific binding sites in survivin full length 3’UTR were mutated (schematic left) and luciferase activity was measured and compared with WT.

miR-214-3p associates with survivin mRNA. A. Schematic representation for constructs of different survivin luciferase reporters containing the full length 3’UTR (FL-3’UTR), CR (F1), or 3’UTR fragments with individual predicted miR-214-3p binding sites (F2-F4). B. Luciferase activity in the different survivin reporter constructs following co-transfection with pre-miR-214-3p (50nM) or control miR in TE7 cells for 36 hours. Firefly luciferase activities were normalized to Renilla luciferase activities and expressed as the mean of three independent experiments, where all experiments were carried out in triplicate. Error bars represent mean ± S.D. and * represents statistically significant p < 0.05, p values based on two-tailed Student's t test. C & D. The binding sequence of the miR-214-3p potential binding site in survivin 3’UTR fragments F2 (C) and F4 (D) were mutated (schematic, left top and bottom) by substituting 4 bases (underlined). Luciferase activity was measured and compared with its WT fragment. E. Binding sequences of both the specific binding sites in survivin full length 3’UTR were mutated (schematic left) and luciferase activity was measured and compared with WT.