Table 2.
Primer sequences used to generate survivin mRNA luciferase reporter constructs for miR-214-3p binding studies.
Name | Sequence | Region | Cutting Site |
---|---|---|---|
pmirGLO-F1-Fwd | GAGCTC CCATTAACCGCCAGATTTGA | 66-550 | Sac I |
pmirGLO-F1-Rev | TCTAGATCAATCCATGGCAGCCAGCT | 66-550 | Xba I |
pmirGLO-F2-Fwd | GAGCTCGCACCACTTCCAGGGTTTAT | 587-1130 | Sac I |
pmirGLO-F2-Rev | TCTAGAAGAGGAGCCAGGGACTCTGT | 587-1130 | Xba I |
pmirGLO-F3-Fwd | GAGCTCCAGAGTCCCTGGCTCCTCTA | 1112-1875 | Sac I |
pmirGLO-F3-Rev | TCTAGAAGCCCGGATGATACAAACAG | 1112-1875 | Xba I |
pmirGLO-F4-Fwd | GAGCTCTTGTATCATCCGGGCTCCT | 1860-2619 | Sac I |
pmirGLO-F4-Rev | TCTAGACATGAGACTTTATTGGCAATTGTG | 1860-2619 | Xba I |
pmirGLO-UTR-Fwd | GAGCTCAGGGAGGAAGAAGGCAGTGT | 843-2617 | Sac I |
pmirGLO-UTR-Rev | TCTAGATGAGACTTTATTGGCAATTGTGAG | 843-2617 | Xba I |
pmirGLO-Mut-F2-Fwd | GTGGACTTGGCAGGTGCCTAACAAATCTGAGCTGCAGGTTCC | 587-1130 | N/A |
pmirGLO-Mut-F2-Rev | GGAACCTGCAGCTCAGATTTGTTAGGCACCTGCCAAGTCCAC | 587/1130 | N/A |
pmirGLO-Mut-F4-Fwd | GAGACAGCAGTGCCCAACACCCAGAAGAGACCAGC | 1860-2619 | N/A |
pmirGLO-Mut-F4-Rev | GCTGGTCTCTTCTGGGTGTTGGGCACTGCTGTCTC | 1860-2619 | N/A |