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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Aug 3;35(16):2087–2097. doi: 10.1038/onc.2015.271

Table 3.

Primer sequences used to generate CUG-BP1 mRNA luciferase reporter constructs for miR-214-3p binding studies.

Name Sequence Region Cutting Site
pmirGLO-F1-Fwd GCTAGCGCAAAGGGTGCTGTTTTGTT 184-660 Nhe I
pmirGLO-F1-Rev GTCGACAAGGTTTCCCCACACAGATG 184-660 Sal I
pmirGLO-F2-Fwd GCTAGCACTCAGGCCTACTCGGGTATC 1085-1424 Nhe I
pmirGLO-F2-Rev GTCGACTAAGCCGCTTCATGCCAAT 1085-1424 Sal I
pmirGLO-F3-Fwd GCTAGCTAAGAGGCCAAGGTGCAATC 3796-4080 Nhe I
pmirGLO-F3-Rev GTCGACGAGCTGAGCCCTTTCACAC 3796-4080 Sal I
pmirGLO-F4-Fwd GCTAGCTCTTCCCTGGAGCGACTCT 5403-5854 Nhe I
pmirGLO-F4-Rev GTCGACAAGCCCCGGTAAGACCTG 5403-5854 Sal I
pmirGLO-F5-Fwd GCTAGC GCCTGGAGAGACACGAGAAG 5896-6296 Nhe I
pmirGLO-F5-Rev GTCGACCCACAAGAGAGGAGGGTCAG 5896-6296 Sal I
pmirGLO-Mut-F1-Fwd CCCTATACAGATGAAACCTGACAACAGTGAGAAGAACAATGTGG 184-660 N/A
pmirGLO-Mut-F1-Rev CCACATTGTTCTTCTCACTGTTGTCAGGTTTCATCTGTATAGGG 184-660 N/A
pmirGLO-Mut-F4-Fwd CCCAGAAAAAAGTTTTGCATGTTTCCTGACATTTCTTCACACCTTCGTATATATCACC 5403-5854 N/A
pmirGLO-Mut-F4-Rev GGTGATATATACGAAGGTGTGAAGAAATGTCAGGAAACATGCAAAACTTTTTTCTGGG 5403-5854 N/A