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. 2015 Oct 3;2(11):1735–1750. doi: 10.1016/j.ebiom.2015.09.049

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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Dehydroascorbic acid and ascorbic acid transport into human and mouse RBCs as a function of time.

Humans RBCs are in grouped Figure A, mouse RBCs are in Figure B. Each grouped figure contains four panels. For each grouped figure: in the left upper panel, RBC ascorbate concentration is shown after incubation with one concentration of dehydroascorbic acid; and in the right upper panel, RBC ascorbate concentration is shown after incubation with the same concentration of ascorbic acid. The lower panels are the corresponding extracellular concentrations: dehydroascorbic acid added on the left lower side, ascorbic acid added on the right lower side. Within each panel, a different symbol represents blood from a different human or mouse, and from each human or mouse three or more independent samples were obtained and analyzed at every time point. All open black and white symbols on both sides indicate sample analyses by HPLC, with no reducing agent added. For color symbols: green symbols (, ) indicate analyses by HPLC, with reducing agent (500 μM TCEP) present during incubation; red symbols (, ) indicate analyses by scintillation spectrometry, without reducing agent added; blue symbols (, ) indicate analyses by scintillation spectrometry, with reducing agent (500 μM TCEP) present during incubation.

The concentration of dehydroascorbic acid/ascorbic acid was 2 μM. For clarity, concentrations and species are shown in each panel. Additional experiments with concentration of 5 μM is shown in supplementary figures. For human RBC experiments, 50 μL RBCs were incubated in 0.5 mL total volume using PBS with 5 mM glucose. For mouse RBC experiments, 30 μL RBCs were incubated in 0.3 mL total volume using PBS with 5 mM glucose. Note that initial concentrations are different in the same figures because animals/human often do not have the same initial values, and some data represent scintillation spectrometry results, where initial values should be close to or at zero. See methods and supplementary Fig. 3 legend for other details.

Inline graphic — Dehydroascorbic acid and ascorbic acid transport into human and mouse RBCs as a function of time.

Humans RBCs are in grouped Figure A, mouse RBCs are in Figure B. Each grouped figure contains four panels. For each grouped figure: in the left upper panel, RBC ascorbate concentration is shown after incubation with one concentration of dehydroascorbic acid; and in the right upper panel, RBC ascorbate concentration is shown after incubation with the same concentration of ascorbic acid. The lower panels are the corresponding extracellular concentrations: dehydroascorbic acid added on the left lower side, ascorbic acid added on the right lower side. Within each panel, a different symbol represents blood from a different human or mouse, and from each human or mouse three or more independent samples were obtained and analyzed at every time point. All open black and white symbols on both sides indicate sample analyses by HPLC, with no reducing agent added. For color symbols: green symbols (, ) indicate analyses by HPLC, with reducing agent (500 μM TCEP) present during incubation; red symbols (, ) indicate analyses by scintillation spectrometry, without reducing agent added; blue symbols (, ) indicate analyses by scintillation spectrometry, with reducing agent (500 μM TCEP) present during incubation.

The concentration of dehydroascorbic acid/ascorbic acid was 2 μM. For clarity, concentrations and species are shown in each panel. Additional experiments with concentration of 5 μM is shown in supplementary figures. For human RBC experiments, 50 μL RBCs were incubated in 0.5 mL total volume using PBS with 5 mM glucose. For mouse RBC experiments, 30 μL RBCs were incubated in 0.3 mL total volume using PBS with 5 mM glucose. Note that initial concentrations are different in the same figures because animals/human often do not have the same initial values, and some data represent scintillation spectrometry results, where initial values should be close to or at zero. See methods and supplementary Fig. 3 legend for other details.