Fig. 5.
Assessment of DTG and haloperidol binding affinities for PGRMC1. Experiments were performed with rat liver membranes. Shown on the left is a representative inhibition curve of [3H]-progesterone binding (at 30 nM) to PGRMC1 in the presence of increasing concentrations of non-radioactive DTG or haloperidol. Nonspecific binding was measured by the addition of 10 μM of nonradioactive progesterone and subtracted, as previously described by Peluso et al. (2008). Inhibition constants (KI) are reported as mean ± SEM from three separate experiments each performed in triplicates for DTG and two separate experiments each performed in triplicates for haloperidol.