Fig. 4.
A comparison of Gardos channel activity in red cells from HbSS and HbSC patients. Red cells were treated as described in the legend to Fig. 2. (a) Gardos channel activity (CLT-sensitive K+ influx in the presence of 100 μM ouabain and 10 μM bumetanide) in fully oxygenated and fully deoxygenated red cells. Histograms represent means ± S.E.M., n = 110 for HbSC patients and n = 40 for HbSS. ***p < 0.001. (b) Pearson correlation between Psickle and Gardos channel in HbSS and HbSC patients. Insert shows a higher resolution at lower magnitudes of activity of Psickle and Gardos channel. Correlations were calculated as r = 0.433 (p < 0.001) for HbSC red cells and r = 0.530 (p < 0.001) for HbSS.