Fig. 8.

KCC activity in density separated red cell fractions from HbSS and HbSC patients. Red cells were density separated using OptiPrep gradients and either kept on ice or treated with nystatin. They then were suspended at 20% Hct in Cl⁻ free saline before being diluted 10-fold into test tubes for measurement of K⁺ influx. KCC was measured as Cl⁻ dependent K⁺ influx in the presence of ouabain (100 μM), bumetanide (10 μM) and CLT (5 μM). (a) HbSS red cells. (b) HbSC red cells. Histograms represent means ± S.E.M., n = 6. *p < 0.05, **p < 0.01 comparing KCC activity in light and dense fractions.