Fig. 3.

Inhibition of rat and human aortic smooth muscle cell proliferation by treatment with JQ1(+).

A and B. Dose responses to JQ1 enantiomers showing inhibition of proliferation of primary rat (A) and human (B) aortic SMCs, by JQ1(+) but not JQ1(−). Proliferation was measured by CellTiter-Glo assay or BrdU assay 24 h after PDGF-BB stimulation. Quantification: mean ± SEM; n = 3 independent experiments.

C. Immunostaining of PCNA showing reduced proliferating cells in JQ1(+)-treated (500 μg per rat) carotid arteries versus vehicle control 14 days after injury. Arrowhead: IEL; arrow: EEL. “Negative staining” refers to the background control without using a primary antibody. Quantification: mean ± SEM; n = 4 animals; *P < 0.05 versus vehicle control. HPF: high power field. Scale bar: 50 μm.