Supplemental Fig. 2.

Mice were chronically exposed to air or CS over a period of 12 weeks and then intranasally challenged with 5 × l0⁴ CFU of S. pneumoniae (Sp) or PBS (Mock). IFN-g levels were measured in the BAL 24 h post-infection (a), and restimulated lung cells. (b). Total cell numbers, and absolute numbers (× 10⁶) of NK cells (CD45⁺ NK1,1⁺ TCRb⁻), NKT cells (CD45⁺ PBS-57 CD1d tetramer⁺ TCRb⁺), conventional T cells (CD45⁺ PBS-57 CD1d tetramer⁺ TCRb⁺ CD4⁺ and/or CD8⁺) were evaluated in lung tissues 24 h post-infection. Activation of NKT and T cells was estimated by CD69 MFI. (c) Results were expressed as mean ± SEM. *: p < 0.05.