Supplemental Fig. 3.

Mice were chronically exposed to air or CS over a period of 12 weeks and then intranasally challenged with 4 × 10⁴ CFU of S. pneumoniae (Sp — black bars) or with PBS (Mock — white bars). Lung tissues were collected 1 day post-infection to sort alveolar macrophages (AM; CD45⁺ Siglec F⁺) and dendritic cells (DC; CD45⁺ Siglec F⁻ la⁺ CD64⁺). mRNA levels of ll-1b, ll-6, Il-12p40 and Il-23pl9 were evaluated in AM (a) and DC (b) compared to GAPDH. One representative experiment out of three independent ones is represented.