Supplemental Fig. 4.

Mice were chronically exposed to air or CS over a period of 12 weeks and then intranasally challenged with 4 × l0⁴ CFU of S. pneumoniae (Sp). Recombinant IL-22 was intranasally given to mice the day before Sp infection. Activation status of AM and DC was assessed by CD86 expression (a). Percentages of NK cells and NKT cells were analyzed among CD45⁺ cells in lung tissues one day post-infection (b). IL-17 and IFN-g levels were evaluated in restimulated lung cells (c). Results were expressed as mean ± SEM. *: p < 0.05 vs controls.