Supplemental Fig. 5.

PBMC were stimulated for 24 h with S. pneumoniae. IL-17 and IL-22 production was analyzed by intracellular staining and flow cytometry analysis, (a) Gating strategy used to identify Lin⁻ cells, NK, NKT, Tγδ, MAIT cells as well as CD4⁺ and CD8⁺ T cells (bold lines), (b) Expression of IL-22 and IFN-y in NK and Lin′ cells after stimulation with S. pneumoniae or with PBS (Mock) in a non-smoker control, a smoker donor and a COPD patient. This is a representative experiment out of at least 10.