Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1969 Mar;22(2):205–211. doi: 10.1136/jcp.22.2.205

Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal

J L Raven 1, M B Robson 1, P L Walker 1, P Barkhan 1
PMCID: PMC474034  PMID: 5776552

Abstract

An improved and simplified method is described for the measurement of vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal. The extraction of serum in the presence of cyanide and the incorporation of B12-deficient serum into the intrinsic factor control has increased the accuracy of the method for both sera and crystalline B12 solutions. There are interesting differences between the results obtained for some sera by the isotope and L. leichmannii methods and the reasons for these differences are discussed.

Full text

PDF
208

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARAKAT R. M., EKINS R. P. Assay of vitamin B12 in blood. A simple method. Lancet. 1961 Jul 1;2(7192):25–26. doi: 10.1016/s0140-6736(61)92711-8. [DOI] [PubMed] [Google Scholar]
  2. Frenkel E. P., Keller S., McCall M. S. Radioisotopic assay of serum vitamin B 12 with the use of DEAE cellulose. J Lab Clin Med. 1966 Sep;68(3):510–522. [PubMed] [Google Scholar]
  3. GROSSOWICZ N., SULITZEANU D., MERZBACH D. Isotopic determination of vitamin B12 binding capacity and concentration. Proc Soc Exp Biol Med. 1962 Mar;109:604–608. doi: 10.3181/00379727-109-27282. [DOI] [PubMed] [Google Scholar]
  4. LAU K. S., GOTTLIEB C., WASSERMAN L. R., HERBERT V. MEASUREMENT OF SERUM VITAMIN B12 LEVEL USING RADIOISOTOPE DILUTION AND COATED CHARCOAL. Blood. 1965 Aug;26:202–214. [PubMed] [Google Scholar]
  5. Matthews D. M., Gunasegaram R., Linnell J. C. Results with radioisotopic assay of serum B12 using serum binding agent. J Clin Pathol. 1967 Sep;20(5):683–686. doi: 10.1136/jcp.20.5.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ROSENTHAL H. L., SARETT H. P. The determination of vitamin B12 activity in human serum. J Biol Chem. 1952 Nov;199(1):433–442. [PubMed] [Google Scholar]
  7. Raven J. L., Walker P. L., Barkhan P. Comparison of the radioisotope dilution-coated charcoal method and a microbiological method (L. leichmannii) for measuring vitamin B12 in serum. J Clin Pathol. 1966 Nov;19(6):610–613. doi: 10.1136/jcp.19.6.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SPRAY G. H. An improved method for the rapid estimation of vitamin B12 in serum. Clin Sci. 1955 Nov;14(4):661–667. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES