Figure 4. Tumour growth in BM transplanted mice.

(a) C1q antigenic levels in reconstituted mice. Levels were measured by ELISA as described in the Methods section 2 months after BM transplant. Results are expressed in μg ml⁻¹, referring to a standard curve derived from a known concentration of purified C1q. (b) Tumour mass measured on various days after tumour cell injection (left graph) and on end-point (day 14) when mice were culled and tumours were removed (right graph). Irradiated recipient mice are represented by a circle (WT) or a triangle (C1qa^−/−). Data are shown as means±s.e.m. Each symbol represents an individual mouse; *P<0.05, **P<0.01, NS, not significant (two-way ANOVA). (c) Representative imagines showing staining for C1q (red) and CD68 (green) in tumour sections of BM transplanted mice at day 14; scale bars, 100 μm. Insets show details of cells (arrowheads) and vessels (arrows) stained for C1q; scale bars, 100 μm.