. 2016 Feb 4;7:16. doi: 10.3389/fimmu.2016.00016

Table 1.

Density, brightness, and diffusion rate obtained from FCS analysis.

Values from FCS		Control L95, GM, U95	IL-2 L95, GM, U95	IFN-α + β L95, GM, U95
Density (N/μm²)	H-2D^d	43.3, 50.5, 58.9	35.6, 42.4, 50.4	45.6, 54.5, 65.0
Density (N/μm²)	Ly49A	3.46, 4.21, 5.11	3.04, 3.79, 4.72	2.78, 3.47, 4.35
Brightness (kHz)	H-2D^d	0.84, 0.99, 1.18	1.17, 1.34, 1.54	0.92, 1.10, 1.32
Brightness (kHz)	Ly49A	7.0, 8.7, 10.7	8.4, 10.5, 13.0	7.9, 10.2, 13.2
Diffusion coefficient (μm²/s)	H-2D^d	0.082, 0.095, 0.110	0.112, 0.145, 0.188	0.110, 0.135, 0.164
Diffusion coefficient (μm²/s)	Ly49A	0.207, 0.242, 0.283	0.233, 0.296, 0.376	0.294, 0.373, 0.472

The bold is to highlight the geometrical mean, in contrast to the confidence interval limits. L95, lower 95% confidence interval limit. GM, geometric mean. U95, upper 95% confidence interval limit. Geometric mean and 95% confidence interval limits calculated from data transformed by the natural logarithm, and thereafter back-calculated to raw data format. Values are not corrected for antibody brightness and binding efficiency. H-2D^d = 55, 48, and 58 cells, Ly49A = 36, 41, and 35 cells, for control, IL-2, and IFN-α + β, respectively. Data pooled from seven independent experiments.