Table 3.

Molecular determinants of PKC-θ localization at the immunological synapse.

Interaction/activity	Molecular determinants	Effect on immunological synapse	Reference
PKC-θ–CD28	Polyproline motif within the PKC-θ V3 hinge region and PYAP motif in CD28; Lck-mediated interaction	PKC-θ V3 hinge and CD28 PYAP motif are required for CD28 cSMAC localization	Kong et al. (124)
PKC-θ–CD28	Sumoylation of PKC-θ at lysines 325 and 506	Abrogated PKC-θ sumoylation reduces PKC-θ localization at the IS and its colocalization with CD28, induces colocalization of PKC-θ and filamin A at periphery of the IS	Wang et al. (139)
PKC-θ–DAG	C1 domains of PKC-θ	C1 domains mediate initial PKC-θ recruitment to the synaptic membrane, but they do not support PKC-θ central accumulation at the synapse	Basu et al. (134), Carrasco and Merida (136), Quann et al. (135)
PKC-θ kinase activity	Unknown, possibly through autophosphorylation at threonine 219 between the tandem C1 domains	PKC-θ kinase activity is required for its recruitment to the IS	Cartwright et al. (138), Thuille et al. (137)
Rltpr	Unknown, no interaction between Rltpr and PKC-θ has been detected	Wild-type Rltpr is required for PKC-θ and CARMA1 recruitment to cSMAC	Liang et al. (113)