Figure 2.

CIH causes a mismatch between preBötC network bursting and the XIIn and disrupts the typically tight input-output relationship. (A) Paired recordings of integrated preBötC and XIIn network rhythms from an individual control slice demonstrating the typical one-to-one burst relationship between premotor rhythm generator and the motor nucleus (top). Following CIH, network activity from the preBötC intermittently fails to produce activity in the XIIn (bottom). Yellow boxes highlight mismatch. Scale Bar: 10 s. (B) Transmission from preBötC to XIIn is reduced following CIH (n_CIH = 16, n_control = 10). Furthermore, the CIH group exhibited a larger variance in rate of intermittent transmission failure compared to the control group (F-ratio = 690, P < 0.001). (C) When transmission from preBötC to XIIn was <90% following CIH, the I/O ratio revealed that CIH disrupts the I/O relationship between the premotor rhythm generator and the motor pool normally observed in control (F-ratio = 51.63, P < 0.0001). (D) Representative poincare maps of the I/O ratios illustrating that CIH also disrupted the normal predictability of I/O relationship. The dispersion in both SD1 and SD2 was different between control and CIH (see text).