Table 2. Primer information for various PCR amplifications in the study of DMI resistance mechanisms in Erysiphe necator.

Primer	Sequence (5’→3’)	Application
L1	TTGTCGACCCCCAAGACTAC	cyp51 sequencing, amplifies section 37–1700, ~1.3 kb amplicon
R900	GACTTGACGCTCCTGTGCTA
L502	CGCCGAAGAGATTTACACTA	cyp51 sequencing, amplifies section 405–1238, ~0.97 kb amplicon
R1000	GATCCCATTTGAGAGGGTCT
L1300	CATGGAAGAGTTGTATGAGGAACA	cyp51 sequencing, amplifies section 1097–1800, ~0.76 kb amplicon
R1300	CAATTCTTCTAACCCTAACACCTG
L419	CAGTCTATCTGGGACTTCAAGG	cyp51 sequencing/verifying codon 136, amplifies section 419–1000, ~0.58 amplicon
R419	AACAGTTCTTTGGGCATGAT
FScyp51 RScyp51	ACTAATTTAACAACTCCGGTCTTTGGA ACTCGACCATTTACGGACCTTTTT	cyp51-specific forward and reverse primers for SNP genotyping
Probe 1	VIC-TTGGACAATCAAATACAAC	mutant allele-specific and wild-type allele-specific probes, SNP genotyping
Probe 2	FAM-TTTGGACAATCATATACAAC
FGcyp51	CATGCGCGAGATCGTTCAC	cyp51-forward and cyp51-reverse primers for gene expression analysis
RGcyp51	CAGAAATGGTTTGCCGAAAGCA
FGtub	TGATTGTCCAAATTCCAAACTCATGGA	tub-forward and tub-reverse primers for gene expression analysis
RGtub	AGGAATGGAACGCTTCAATGGT
Pcyp51	FAM-AAGAGCCGTTTTCATAAACTTT	Encyp51 probe, for gene expression analysis
Ptub	FAM-CCAATGCGGAAATCAA	Entub probe, for gene expression analysis