Skip to main content
. 2016 Feb 3;12(2):e1005416. doi: 10.1371/journal.ppat.1005416

Fig 5. PrPSc containing M at residue 129 is underrepresented in most heterozygous (MV) iCJD patients.

Fig 5

(a) Molecular typing of PrPSc from the brain homogenate of heterozygous iCJD cases 15–20 by the method of Parchi et al. [33]. Each sample (middle lane) is shown flanked by Type 1 (left lane) and Type 2 (right lane) reference standards from sCJD MM1 and VV2 subtype cases, respectively. Case number and brain region are indicated above each blot while PrPSc type is indicated below. Protease-resistant PrP from all samples was of the A glycotype where diglycosylated PrP ≤ monoglycosylated PrP [33]. The most informative exposure for the test and reference standard samples from a series of timed exposures is shown. (b) Relative abundance of PrPSc-M129 in MV2 iCJD. Data are the percent mean ± SD of PrPSc-M129 (black bars) detected in the CC of iCJD patients. A stoichiometrically adjusted solution of 50% rHuPrP-M129 and 50% rHuPrP-V129 (rPrP (50%), hatched bar) was used as a control (n = 8). Each bar represents n = 3–4 technical replicates. Statistical analysis was done using a 1-way ANOVA with Dunnett’s post-test using rPrP (50%) as the control sample. **p = 0.001 to 0.01.