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. 2016 Feb 3;11(2):e0147683. doi: 10.1371/journal.pone.0147683

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© 2016 Tagliaferri et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) (I) The percentage of ES^Zscan4_Em cells was assessed on ESCs cultured for 3 days in: RM+DMSO; RM supplemented with RA; RA plus BMS493; BMS753, and UVI2060. The mean % ES^Zscan4_Em cells ± SD of two independent experiments is shown. (II) The mRNA expression levels were assessed on ESCs treated with RA and RA plus BMS493 by qRT-PCR and normalized to RM+DMSO condition. The average and SD of duplicate samples from each of three independent biological replicates are shown: *, p < .05; **, p < .01; ***, p < .001, in a Student’s t test. B (I) The mRNA expression levels were assessed on ESCs treated with BMS753 by qRT-PCR and normalized to RM+DMSO condition. The average and SD of duplicate samples from each of three independent biological replicates are shown: **, p < .01; ***, p < .001, in a Student’s t test. (II) The mRNA expression levels were assessed on ESCs treated with UVI2060 by qRT-PCR and normalized to RM+DMSO condition. The average and SD of duplicate samples from each of three independent biological replicates are shown: *, p < .05; ***, p < .001, in a Student’s t test.