Fig 4. RA-Zscan4⁺ cells molecular and cellular characterization.

(A) The global gene expression profiles between RA-Zscan4⁺ and RA-Zscan4^- cell populations by DNA chip microarray hybridization. List of RA-Zscan4⁺ probes upregulated more than 20-fold compared to RA-Zscan4^-. (B) RA-Zscan4⁺ cells expression versus RM-Zscan4⁺. (C) RA-Zscan4⁺ and RA-Zscan4^- cells were collected, separated by FACS and plated in RM. The mean % RA-Zscan4⁺ or RA-Zscan4^- cells ± SD of three independent experiments is presented with statistical analysis performed using Student’s t test (**, p < .01; ***, p < .001). (D) RA-Zscan4⁺ and RA-Zscan4^- cells were collected and separated by FACS, plated in RM and were characterized based on AP-positive colonies after 5 days in RM (n = 3).