Fig. 10.

Effect of exogenous, human recombinant (rh) forms of IL-6 and TGF-β1 on the nuclear binding of HIF-1α, NF-κB, and AP-1 in ovarian cancer cells (a–d A2780; e–h OVCAR-3; i–l SKOV-3). Panels a, c, e, g, i, k show the results of dose–response experiments, while panels b, d, f, h, j, l represent the results of the time-course studies. Doses of recombinant proteins applied on the cancer cells (for 24 h) in the dose–response experiments correspond to a wide range of concentrations of a certain protein determined in conditioned medium generated by senescent HPMCs. In the time-course experiments, the lowest dose capable of inducing a given transcription factor was used. The asterisks indicate significant differences as compared with the control, untreated cells (100 %). The experiments were performed in hexaplicates