Fig. 2.

Modulatory effect of HPMCs on ovarian cancer cell-dependent angiogenic activity of endothelial cells in vitro. Both proliferation (PROL) and migration (MIGR) of endothelial cells (HUVECs) were examined in response to their exposure (24 h) to CM harvested from A2780 (a), OVCAR-3 (b), and SKOV-3 (c) ovarian cancer cells which were pre-incubated with CM from young and senescent HPMCs, or in response to exposure to CM generated by A2780 (d), OVCAR-3 (e), and SKOV-3 cells (f) co-cultured with young and senescent HPMCs. Single asterisks indicate a significant difference as compared with cells subjected to CM from cancer cells pre-incubated with CM from young HPMCs. Double asterisks indicate a significant difference as compared with cells subjected to CM from cancer cells pre-incubated with autologous CM (treated as 100 %). Experiments were performed with HPMCs from 8 different donors whose age ranged from 34 to 38 years old (samples were not pooled). Ovarian cancer cells were used in hexaplicates. Endothelial cells were used in duplicates