Fig. 5.

Magnitude of angiogenesis in tumors that developed upon i.p. injection of ovarian cancer cells alone or in combination with young and senescent HPMCs. The results derive from experiments as shown and described in Fig. 4. Representative images showing the results of immunostaining for markers of the vascular endothelium, CD31 (a) and CD34 (c). CD31/CD34-positive cells display brown membrane and/or cytoplasm staining (some examples are marked with arrows). Quantification of the brown-stained area reflecting the presence of CD31- (b) and CD34-positive cells (d). The results are expressed as a percentage, and the whole area of a specimen is considered as 100 %. The single asterisks indicate a significant difference as compared with the xenografts that developed in the presence of young HPMCs. The double asterisks indicate a significant difference as compared with the xenografts that developed in the absence of HPMCs. Experiments were performed with HPMCs from 6 different donors and with 6 pairs of animals (each pair received cancer cells and young or senescent HPMCs from the same donor)