Fig. 7.

Effect of exogenous, recombinant forms of human IL-6 and TGF-β1 on ovarian cancer cell-dependent angiogenic reactions of endothelial cells. Both proliferation (PROL) and migration (MIGR) of endothelial cells (HUVECs) were examined in response to CM from A2780 (a, d), OVCAR-3 (b, e), and SKOV-3 cells (c, f) which were pre-incubated with recombinant human (rh) IL-6 (5 ng/ml) and TGF-β1 (1 ng/ml). Asterisks indicate a significant difference as compared with endothelial cells subjected to CM from cancer cells not exposed to rhIL-6 or rhTGF-β1 (treated as 100 %). Experiments were performed with CM obtained from 6 separate cultures of ovarian cancer cells (for each cell line). Endothelial cells were used in duplicates