Fig. 8.

Activation of transcription factors involved in angiogenic endothelial cell reactions in ovarian cancer cells in response to senescence of HPMCs. Ovarian cancer cell lines A2780 (a), OVCAR-3 (b), and SKOV-3 (c) were subjected to conditioned medium from young and senescent HPMCs (24 h) and then the activation of HIF-1α, NF-κB, and AP-1 in the cancer cells was examined. Single asterisks indicate a significant difference as compared with cells exposed to CM from young HPMCs. Double asterisks indicate a significant difference as compared with cells subjected to autologous CM (treated as 100 %). Experiments were performed with HPMCs from 8 different donors (samples were not pooled). Ovarian cancer cells were used in hexaplicates