Table 3.

Secretion of pro-angiogenic agents by ovarian cancer cells in response to a conditioned medium generated by senescent HPMCs upon its pre-incubation with antibodies neutralizing IL-6 and TGF-β1

Neutralized mediator	A2780		OVCAR-3		SKOV-3
	HGF	VEGF	CXCL1	CXCL8	CXCL1	CXCL8	VEGF
IL-6	88 ± 3*	72 ± 4*	91 ± 2*	64 ± 3*	81 ± 2*	66 ± 3*	72 ± 1*
TGF-β1	76 ± 3*	82 ± 1*	78 ± 2*	76 ± 2*	80 ± 5*	71 ± 2*	67 ± 8*

Samples of CM from senescent HPMCs were pre-incubated with antibodies against IL-6 (200 ng/ml) and TGF-β1 (400 ng/ml) for 24 h. Afterwards, the media were added to ovarian cancer cells for 24 h. After incubation, the media were removed and the cells were exposed to fresh serum-free media for 24 h to generate autologous CM in which the angiogenic agent level was quantified. The values are expressed as a percentage of the control cells (cancer cells exposed to CM from senescent HPMCs without IL-6/TGF-β1 neutralization are considered as 100 %). The results derive from experiments performed with CM produced by HPMCs from 6 different donors. Cancer cells were used in hexaplicates. The asterisks indicate a significant difference as compared with the control group