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. 2016 Feb 4;6:20047. doi: 10.1038/srep20047

Figure 5. Pilus Island 1 is a hotspot for integration of MGEs carrying antimicrobial genes.

Figure 5

(a) Chromosomal macrolide resistance was encoded by ermTR in a single strain. The top schematic shows ermTR gene (green) carried by a MGE which has inserted upstream of PI-1 in strain NGBS417. Depicted in the middle schematic is the region between nucleotides 589,950 and 619,400 (from the beginning of gene aroA to the end of gene cylD) in the genome of reference strain NGBS128. This region contains PI-1 genes, comprising the regulator rofA (orange), major and minor pilin subunits (purple), and three class C sortases (green). (b) Loss of PI-1 is associated with acquisition of an MGE carrying multiple resistance genes in several CC17 isolates. The bottom schematic shows that in 12 CC17 strains, PI-1 has been exchanged with a >70 kbp MGE carrying multiple antibiotic resistance genes. Conserved genes are indicated in blue, variable genes are indicated in grey. Antimicrobial resistance genes are indicated. Predicted function of genes is shown in Table S5.