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. 2016 Feb 4;6:20184. doi: 10.1038/srep20184

Figure 1. Chromatographic separation of protein content in courtship, male and female water.

Figure 1

(a) RP-HPLC elution profile of courtship water (n = 5 courting couples) shows several courtship-specific peaks that are absent in male and female water. A silver-stained SDS-PAGE gel of fractions I to V reveals the presence of multiple 20–25 kDa proteins. (b) RP-HPLC elution profile of male (n = 5) and (c) female (n = 5) water with silver-stained SDS-PAGE gel. A 20 kDa band appears in fractions III to V of male water. The acetonitrile gradient (0% to 80% ACN in 0.1% TFA) is represented by a grey line in all elution profiles.